maxiscript sp6 rna transcription kit Search Results


sp6 rna polymerase  (New England Biolabs)
96
New England Biolabs sp6 rna polymerase
Sp6 Rna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/maxiscript+sp6+rna+transcription+kit/SP6+RNA+Polymerase/custom%40m0207%4019556782
Average 96 stars, based on 1 article reviews
sp6 rna polymerase - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier
direct-zol rna microprep  (Zymo Research)
99
Zymo Research direct-zol rna microprep
Direct Zol Rna Microprep, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/maxiscript+sp6+rna+transcription+kit/Direct-zol+RNA+Microprep/custom%40r2060%4039261662
Average 99 stars, based on 1 article reviews
direct-zol rna microprep - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier
pgemt  (Promega)
90
Promega pgemt
Pgemt, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/maxiscript+sp6+rna+transcription+kit/pgem+t+easy/pmc01563885-192-9-11
Average 90 stars, based on 1 article reviews
pgemt - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
template dna  (Thermo Fisher)
99
Thermo Fisher template dna
The hζ-globin mRNA 3′UTR contains a short poly(A) tail. (A) Measurement of poly(A) tail length (An) on transgenic globin mRNAs. Site-specific RNase H cleavage of globin mRNAs was targeted by <t>an</t> <t>antisense</t> <t>DNA</t> oligomer (horizontal bar) complementary to a sequence located approximately 100 nt 5′ to its poly(A) addition site. The RNA fragments were resolved on a denaturing acrylamide gel and electrotransferred to a nylon membrane. The 3′-terminal fragment, which contains the poly(A) tail, was visualized by hybridization with a site-specific 32P-labeled 3′UTR DNA probe, the position of which is indicated (∗□). The length of the fully deadenylated 3′-terminal RNA fragment was established by addition of excess oligo(dT) to the hybridization reaction prior to the RNase H digestion. (B) The hζ and α3′ζ mRNAs have shortened poly(A) tails. The poly(A) tail lengths of hα-, hζ-, and chimeric α3′ζ-globin mRNAs from transgenic reticulocytes were determined with the RNase H assay. The positions of the fully deadenylated 3′-terminal fragments are aligned, and the positions equivalent to a poly(A) tail length of 40 residues (determined by DNA size markers electrophoresed in parallel for each study) are indicated by an arrow. The results for each mRNA were confirmed in experiments with reticulocytes from mice representing two or more independent transgenic lines. (C) Densitometric analyses of the poly(A) tails from panel B. Signal intensities from hα-, hζ-, and chimeric α3′ζ-globin poly(A) tails from panel B were quantitated by scanning densitometry, and the adjusted scans were aligned to a standard scale to facilitate direct comparison.
Template Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/maxiscript+sp6+rna+transcription+kit/DNA/pmc00121457-142-15-25
Average 99 stars, based on 1 article reviews
template dna - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier
hindiii  (New England Biolabs)
99
New England Biolabs hindiii
The hζ-globin mRNA 3′UTR contains a short poly(A) tail. (A) Measurement of poly(A) tail length (An) on transgenic globin mRNAs. Site-specific RNase H cleavage of globin mRNAs was targeted by <t>an</t> <t>antisense</t> <t>DNA</t> oligomer (horizontal bar) complementary to a sequence located approximately 100 nt 5′ to its poly(A) addition site. The RNA fragments were resolved on a denaturing acrylamide gel and electrotransferred to a nylon membrane. The 3′-terminal fragment, which contains the poly(A) tail, was visualized by hybridization with a site-specific 32P-labeled 3′UTR DNA probe, the position of which is indicated (∗□). The length of the fully deadenylated 3′-terminal RNA fragment was established by addition of excess oligo(dT) to the hybridization reaction prior to the RNase H digestion. (B) The hζ and α3′ζ mRNAs have shortened poly(A) tails. The poly(A) tail lengths of hα-, hζ-, and chimeric α3′ζ-globin mRNAs from transgenic reticulocytes were determined with the RNase H assay. The positions of the fully deadenylated 3′-terminal fragments are aligned, and the positions equivalent to a poly(A) tail length of 40 residues (determined by DNA size markers electrophoresed in parallel for each study) are indicated by an arrow. The results for each mRNA were confirmed in experiments with reticulocytes from mice representing two or more independent transgenic lines. (C) Densitometric analyses of the poly(A) tails from panel B. Signal intensities from hα-, hζ-, and chimeric α3′ζ-globin poly(A) tails from panel B were quantitated by scanning densitometry, and the adjusted scans were aligned to a standard scale to facilitate direct comparison.
Hindiii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/maxiscript+sp6+rna+transcription+kit/HindIII/custom%40r0104%4010%2E1038%2Fs41563-019-0368-6
Average 99 stars, based on 1 article reviews
hindiii - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier
ecori  (New England Biolabs)
99
New England Biolabs ecori
The hζ-globin mRNA 3′UTR contains a short poly(A) tail. (A) Measurement of poly(A) tail length (An) on transgenic globin mRNAs. Site-specific RNase H cleavage of globin mRNAs was targeted by <t>an</t> <t>antisense</t> <t>DNA</t> oligomer (horizontal bar) complementary to a sequence located approximately 100 nt 5′ to its poly(A) addition site. The RNA fragments were resolved on a denaturing acrylamide gel and electrotransferred to a nylon membrane. The 3′-terminal fragment, which contains the poly(A) tail, was visualized by hybridization with a site-specific 32P-labeled 3′UTR DNA probe, the position of which is indicated (∗□). The length of the fully deadenylated 3′-terminal RNA fragment was established by addition of excess oligo(dT) to the hybridization reaction prior to the RNase H digestion. (B) The hζ and α3′ζ mRNAs have shortened poly(A) tails. The poly(A) tail lengths of hα-, hζ-, and chimeric α3′ζ-globin mRNAs from transgenic reticulocytes were determined with the RNase H assay. The positions of the fully deadenylated 3′-terminal fragments are aligned, and the positions equivalent to a poly(A) tail length of 40 residues (determined by DNA size markers electrophoresed in parallel for each study) are indicated by an arrow. The results for each mRNA were confirmed in experiments with reticulocytes from mice representing two or more independent transgenic lines. (C) Densitometric analyses of the poly(A) tails from panel B. Signal intensities from hα-, hζ-, and chimeric α3′ζ-globin poly(A) tails from panel B were quantitated by scanning densitometry, and the adjusted scans were aligned to a standard scale to facilitate direct comparison.
Ecori, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/maxiscript+sp6+rna+transcription+kit/EcoRI/custom%40r0101%4010%2E1038%2Fs41563-019-0368-6
Average 99 stars, based on 1 article reviews
ecori - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier
32p]utp  (ICN Pharmaceuticals)
90
ICN Pharmaceuticals 32p]utp
The hζ-globin mRNA 3′UTR contains a short poly(A) tail. (A) Measurement of poly(A) tail length (An) on transgenic globin mRNAs. Site-specific RNase H cleavage of globin mRNAs was targeted by <t>an</t> <t>antisense</t> <t>DNA</t> oligomer (horizontal bar) complementary to a sequence located approximately 100 nt 5′ to its poly(A) addition site. The RNA fragments were resolved on a denaturing acrylamide gel and electrotransferred to a nylon membrane. The 3′-terminal fragment, which contains the poly(A) tail, was visualized by hybridization with a site-specific 32P-labeled 3′UTR DNA probe, the position of which is indicated (∗□). The length of the fully deadenylated 3′-terminal RNA fragment was established by addition of excess oligo(dT) to the hybridization reaction prior to the RNase H digestion. (B) The hζ and α3′ζ mRNAs have shortened poly(A) tails. The poly(A) tail lengths of hα-, hζ-, and chimeric α3′ζ-globin mRNAs from transgenic reticulocytes were determined with the RNase H assay. The positions of the fully deadenylated 3′-terminal fragments are aligned, and the positions equivalent to a poly(A) tail length of 40 residues (determined by DNA size markers electrophoresed in parallel for each study) are indicated by an arrow. The results for each mRNA were confirmed in experiments with reticulocytes from mice representing two or more independent transgenic lines. (C) Densitometric analyses of the poly(A) tails from panel B. Signal intensities from hα-, hζ-, and chimeric α3′ζ-globin poly(A) tails from panel B were quantitated by scanning densitometry, and the adjusted scans were aligned to a standard scale to facilitate direct comparison.
32p]Utp, supplied by ICN Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/maxiscript+sp6+rna+transcription+kit/g+32p+atp/10__1074_slash_jbc__m210526200-92-18-19
Average 90 stars, based on 1 article reviews
32p]utp - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
hiscribe t7 high yield rna synthesis kit  (New England Biolabs)
99
New England Biolabs hiscribe t7 high yield rna synthesis kit
The hζ-globin mRNA 3′UTR contains a short poly(A) tail. (A) Measurement of poly(A) tail length (An) on transgenic globin mRNAs. Site-specific RNase H cleavage of globin mRNAs was targeted by <t>an</t> <t>antisense</t> <t>DNA</t> oligomer (horizontal bar) complementary to a sequence located approximately 100 nt 5′ to its poly(A) addition site. The RNA fragments were resolved on a denaturing acrylamide gel and electrotransferred to a nylon membrane. The 3′-terminal fragment, which contains the poly(A) tail, was visualized by hybridization with a site-specific 32P-labeled 3′UTR DNA probe, the position of which is indicated (∗□). The length of the fully deadenylated 3′-terminal RNA fragment was established by addition of excess oligo(dT) to the hybridization reaction prior to the RNase H digestion. (B) The hζ and α3′ζ mRNAs have shortened poly(A) tails. The poly(A) tail lengths of hα-, hζ-, and chimeric α3′ζ-globin mRNAs from transgenic reticulocytes were determined with the RNase H assay. The positions of the fully deadenylated 3′-terminal fragments are aligned, and the positions equivalent to a poly(A) tail length of 40 residues (determined by DNA size markers electrophoresed in parallel for each study) are indicated by an arrow. The results for each mRNA were confirmed in experiments with reticulocytes from mice representing two or more independent transgenic lines. (C) Densitometric analyses of the poly(A) tails from panel B. Signal intensities from hα-, hζ-, and chimeric α3′ζ-globin poly(A) tails from panel B were quantitated by scanning densitometry, and the adjusted scans were aligned to a standard scale to facilitate direct comparison.
Hiscribe T7 High Yield Rna Synthesis Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/maxiscript+sp6+rna+transcription+kit/HiScribe+T7+High+Yield+RNA+Synthesis+Kit/custom%40e2040%4027389988
Average 99 stars, based on 1 article reviews
hiscribe t7 high yield rna synthesis kit - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier
columns  (Cytiva Europe)
94
Cytiva Europe columns
The hζ-globin mRNA 3′UTR contains a short poly(A) tail. (A) Measurement of poly(A) tail length (An) on transgenic globin mRNAs. Site-specific RNase H cleavage of globin mRNAs was targeted by <t>an</t> <t>antisense</t> <t>DNA</t> oligomer (horizontal bar) complementary to a sequence located approximately 100 nt 5′ to its poly(A) addition site. The RNA fragments were resolved on a denaturing acrylamide gel and electrotransferred to a nylon membrane. The 3′-terminal fragment, which contains the poly(A) tail, was visualized by hybridization with a site-specific 32P-labeled 3′UTR DNA probe, the position of which is indicated (∗□). The length of the fully deadenylated 3′-terminal RNA fragment was established by addition of excess oligo(dT) to the hybridization reaction prior to the RNase H digestion. (B) The hζ and α3′ζ mRNAs have shortened poly(A) tails. The poly(A) tail lengths of hα-, hζ-, and chimeric α3′ζ-globin mRNAs from transgenic reticulocytes were determined with the RNase H assay. The positions of the fully deadenylated 3′-terminal fragments are aligned, and the positions equivalent to a poly(A) tail length of 40 residues (determined by DNA size markers electrophoresed in parallel for each study) are indicated by an arrow. The results for each mRNA were confirmed in experiments with reticulocytes from mice representing two or more independent transgenic lines. (C) Densitometric analyses of the poly(A) tails from panel B. Signal intensities from hα-, hζ-, and chimeric α3′ζ-globin poly(A) tails from panel B were quantitated by scanning densitometry, and the adjusted scans were aligned to a standard scale to facilitate direct comparison.
Columns, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/maxiscript+sp6+rna+transcription+kit/Column+Stand+140%2F200+Columns/pmc04552638-248-30-31
Average 94 stars, based on 1 article reviews
columns - by Bioz Stars, 2026-09
94/100 stars
  Buy from Supplier
t7 rna polymerase  (New England Biolabs)
98
New England Biolabs t7 rna polymerase
The hζ-globin mRNA 3′UTR contains a short poly(A) tail. (A) Measurement of poly(A) tail length (An) on transgenic globin mRNAs. Site-specific RNase H cleavage of globin mRNAs was targeted by <t>an</t> <t>antisense</t> <t>DNA</t> oligomer (horizontal bar) complementary to a sequence located approximately 100 nt 5′ to its poly(A) addition site. The RNA fragments were resolved on a denaturing acrylamide gel and electrotransferred to a nylon membrane. The 3′-terminal fragment, which contains the poly(A) tail, was visualized by hybridization with a site-specific 32P-labeled 3′UTR DNA probe, the position of which is indicated (∗□). The length of the fully deadenylated 3′-terminal RNA fragment was established by addition of excess oligo(dT) to the hybridization reaction prior to the RNase H digestion. (B) The hζ and α3′ζ mRNAs have shortened poly(A) tails. The poly(A) tail lengths of hα-, hζ-, and chimeric α3′ζ-globin mRNAs from transgenic reticulocytes were determined with the RNase H assay. The positions of the fully deadenylated 3′-terminal fragments are aligned, and the positions equivalent to a poly(A) tail length of 40 residues (determined by DNA size markers electrophoresed in parallel for each study) are indicated by an arrow. The results for each mRNA were confirmed in experiments with reticulocytes from mice representing two or more independent transgenic lines. (C) Densitometric analyses of the poly(A) tails from panel B. Signal intensities from hα-, hζ-, and chimeric α3′ζ-globin poly(A) tails from panel B were quantitated by scanning densitometry, and the adjusted scans were aligned to a standard scale to facilitate direct comparison.
T7 Rna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/maxiscript+sp6+rna+transcription+kit/T7+RNA+Polymerase/custom%40m0251%4029272705
Average 98 stars, based on 1 article reviews
t7 rna polymerase - by Bioz Stars, 2026-09
98/100 stars
  Buy from Supplier
dnase i  (New England Biolabs)
99
New England Biolabs dnase i
The hζ-globin mRNA 3′UTR contains a short poly(A) tail. (A) Measurement of poly(A) tail length (An) on transgenic globin mRNAs. Site-specific RNase H cleavage of globin mRNAs was targeted by <t>an</t> <t>antisense</t> <t>DNA</t> oligomer (horizontal bar) complementary to a sequence located approximately 100 nt 5′ to its poly(A) addition site. The RNA fragments were resolved on a denaturing acrylamide gel and electrotransferred to a nylon membrane. The 3′-terminal fragment, which contains the poly(A) tail, was visualized by hybridization with a site-specific 32P-labeled 3′UTR DNA probe, the position of which is indicated (∗□). The length of the fully deadenylated 3′-terminal RNA fragment was established by addition of excess oligo(dT) to the hybridization reaction prior to the RNase H digestion. (B) The hζ and α3′ζ mRNAs have shortened poly(A) tails. The poly(A) tail lengths of hα-, hζ-, and chimeric α3′ζ-globin mRNAs from transgenic reticulocytes were determined with the RNase H assay. The positions of the fully deadenylated 3′-terminal fragments are aligned, and the positions equivalent to a poly(A) tail length of 40 residues (determined by DNA size markers electrophoresed in parallel for each study) are indicated by an arrow. The results for each mRNA were confirmed in experiments with reticulocytes from mice representing two or more independent transgenic lines. (C) Densitometric analyses of the poly(A) tails from panel B. Signal intensities from hα-, hζ-, and chimeric α3′ζ-globin poly(A) tails from panel B were quantitated by scanning densitometry, and the adjusted scans were aligned to a standard scale to facilitate direct comparison.
Dnase I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/maxiscript+sp6+rna+transcription+kit/DNase+I/custom%40m0303%4011907228
Average 99 stars, based on 1 article reviews
dnase i - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier

Image Search Results


The hζ-globin mRNA 3′UTR contains a short poly(A) tail. (A) Measurement of poly(A) tail length (An) on transgenic globin mRNAs. Site-specific RNase H cleavage of globin mRNAs was targeted by an antisense DNA oligomer (horizontal bar) complementary to a sequence located approximately 100 nt 5′ to its poly(A) addition site. The RNA fragments were resolved on a denaturing acrylamide gel and electrotransferred to a nylon membrane. The 3′-terminal fragment, which contains the poly(A) tail, was visualized by hybridization with a site-specific 32P-labeled 3′UTR DNA probe, the position of which is indicated (∗□). The length of the fully deadenylated 3′-terminal RNA fragment was established by addition of excess oligo(dT) to the hybridization reaction prior to the RNase H digestion. (B) The hζ and α3′ζ mRNAs have shortened poly(A) tails. The poly(A) tail lengths of hα-, hζ-, and chimeric α3′ζ-globin mRNAs from transgenic reticulocytes were determined with the RNase H assay. The positions of the fully deadenylated 3′-terminal fragments are aligned, and the positions equivalent to a poly(A) tail length of 40 residues (determined by DNA size markers electrophoresed in parallel for each study) are indicated by an arrow. The results for each mRNA were confirmed in experiments with reticulocytes from mice representing two or more independent transgenic lines. (C) Densitometric analyses of the poly(A) tails from panel B. Signal intensities from hα-, hζ-, and chimeric α3′ζ-globin poly(A) tails from panel B were quantitated by scanning densitometry, and the adjusted scans were aligned to a standard scale to facilitate direct comparison.

Journal:

Article Title: Sequence Divergence in the 3? Untranslated Regions of Human ?- and ?-Globin mRNAs Mediates a Difference in Their Stabilities and Contributes to Efficient ?-to-? Gene Developmental Switching

doi:

Figure Lengend Snippet: The hζ-globin mRNA 3′UTR contains a short poly(A) tail. (A) Measurement of poly(A) tail length (An) on transgenic globin mRNAs. Site-specific RNase H cleavage of globin mRNAs was targeted by an antisense DNA oligomer (horizontal bar) complementary to a sequence located approximately 100 nt 5′ to its poly(A) addition site. The RNA fragments were resolved on a denaturing acrylamide gel and electrotransferred to a nylon membrane. The 3′-terminal fragment, which contains the poly(A) tail, was visualized by hybridization with a site-specific 32P-labeled 3′UTR DNA probe, the position of which is indicated (∗□). The length of the fully deadenylated 3′-terminal RNA fragment was established by addition of excess oligo(dT) to the hybridization reaction prior to the RNase H digestion. (B) The hζ and α3′ζ mRNAs have shortened poly(A) tails. The poly(A) tail lengths of hα-, hζ-, and chimeric α3′ζ-globin mRNAs from transgenic reticulocytes were determined with the RNase H assay. The positions of the fully deadenylated 3′-terminal fragments are aligned, and the positions equivalent to a poly(A) tail length of 40 residues (determined by DNA size markers electrophoresed in parallel for each study) are indicated by an arrow. The results for each mRNA were confirmed in experiments with reticulocytes from mice representing two or more independent transgenic lines. (C) Densitometric analyses of the poly(A) tails from panel B. Signal intensities from hα-, hζ-, and chimeric α3′ζ-globin poly(A) tails from panel B were quantitated by scanning densitometry, and the adjusted scans were aligned to a standard scale to facilitate direct comparison.

Article Snippet: Internally 32 P-labeled antisense-oriented RNA probes for RNase protection assays were transcribed in vitro from template DNA by using SP6 RNA polymerase (SP6 Maxiscript kit; Ambion).

Techniques: Transgenic Assay, Sequencing, Acrylamide Gel Assay, Hybridization, Labeling, Rnase H Assay